Validation of chronic lung infection with P.aeruginosa for characterization of novel MvfR inhibitors
The bacterium P. aeruginosa permanently colonizes cystic fibrosis (CF) lungs despite the aggressive antibiotic treatment. This suggested that P. aeruginosa might exist as biofilms- structured communities of bacteria encased in a self-produced polymeric matrix- in chronic CF lung; it uses extracellular quorum-sensing signals to coordinate biofilm formation.
In an effort to characterize novel inhibitors of multivirulence factor regulator (MvfR) the need to develop a chronic lung infection model became apparent. Clinical isolates from sputum of chronic cystic fibrosis patients have been characterized by measuring QS molecules levels and biofilm biomass in vitro. Among these strains, ACC00901 was identified as QS molecule and biofilm hyperproducer strain comparable with PA01 and selected to set-up a chronic lung infection model rat by loading bacteria into agar beads.
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